Fig. 3

Densitometry analysis of microglia, amyloid-beta (Aβ) plaques, and MR1 in the CA1 region of the hippocampus in 5XFAD mice. A Representative images highlighting amyloid beta pathology shows Dapi (blue), IBA1-labeled microglia/macrophages (red), Aβ (green), MR1 (white), and the images merged. Scale bar = 250 µm. B–D Densitometry analysis presented as the relative mean pixel intensity for Aβ (B), IBA1 (C), and MR1 (D). E, F Graphs for the correlational analysis of the densitometry results between MR1 and Aβ show 5XFAD (squares) and 5XFAD/MR1 KO (upside-down triangles) (E), MR1 and IBA1 (F), and IBA1 and Aβ (G). Statistical analysis was performed using a two-way ANOVA with Tukey post hoc multiple comparison test (B–D) or a simple linear regression analysis (E–G). **P < 0.01, ***P < 0.001, ****P < 0.0001 (B–D: n = 4–6/group; E–G: n = 17–21/group). The data are presented as the mean ± standard error of the mean