Fig. 3

The biological functions of MS4A7-s on GAMs and co-cultured GBM cells proved by in vitro experiments. A, B RT-PCR with dot-plot statistical chart verifies MS4A7 isoforms expression in the empty vehicle-control (EV-control), MS4A7-s overexpression (MS4A7-s OE) and MS4A7-l overexpression (MS4A7-l OE) HMC3 cells. C HMC3 cell viability was measured by a CCK8 assay after MS4A7-s OE and MS4A7-l OE compared with the EV-control group on day 5. D U87MG cell viability was measured by a CCK8 assay after co-cultured with EV-control, MS4A7-s OE and MS4A7-l OE HMC3 cells on day 3. E EdU incorporation assay was used to detect the proliferation ability of co-cultured U87MG cells. F Quantitative histograms of EdU incorporation assay. G Phagocytosis assay by co-culturing the EV-control, MS4A7-s OE and MS4A7-l OE HMC3 cells with latex IgG beads. H Quantitative histograms of phagocytosis assay. Data are presented as mean ± SD (n = 3/group). Statistical significances were analyzed by one-way ANOVA (C, D, F, H). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001