Fig. 3

Nicotine-elicited reactive microglia do not exhibit an inflammatory transcripts or phenotypes. A Workflow diagram and the scheme of flow cytometry and cell sorting. SSC: side scatter; FSC: forward scatter; FSC-H: forward scatter-height; FSC-A: forward scatter-area. B qPCR analysis of TNF-α, IL-1β, IL-6, Arg-1 and iNOS mRNA from wild-type mice on day-28 post-administration of l-tartaric acid or nicotine. C Representative images (left) and intensity of ROI cells in the ACC (right). ROI (region of interest), Arg-1 and Iba-1 double positive cells. Scale bar, 20 μm. D Representative images (left) and intensity of ROI cells in the ACC (right). ROI (region of interest), iNOS and Iba-1 double positive cells. Scale bar, 20 μm. Data were presented as mean ± SEM or Median (IQR). *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant. Details of the statistical analyses are presented in Additional file 1: Table S1