Fig. 6
From: The cervical lymph node contributes to peripheral inflammation related to Parkinson’s disease
α-Synuclein aggregate elicited severe ER stress in the macrophage. A Western blot to assess the levels of p-IRE1α, IRE1α, GRP78, ATF6, ATF6-p, ATF4, sXBP-1, p-eIF2α, eIF2α and α-synuclein in BMDMs treated with vehicle control, 100 nM α-synuclein monomer or 100 nM α-synuclein oligomer. B–J Quantitative analysis of the level of ATF6, ATF6-p, GRP78, IRE1α, p-IRE1α, p-eIF2α, eIF2α, sXBP-1, and ATF4 in BMDMs treated with vehicle control, α-synuclein monomer or α-synuclein oligomer. N = 3 independent experiments in each group. K Quantitative analysis of Ddit3 mRNA levels in BMDMs treated with vehicle control, α-synuclein monomer or α-synuclein oligomer using qPCR. N = 3 independent experiments in each group. L Interaction between α-syn and GRP78 was examined by co-IP in RAW 264.7 cells. M Co-localization of α-syn (green) and GRP78 (red) in RAW 264.7 cells after treatment with α-syn. Scale bar, 50 μm. Values are means ± S.E.M, one-way ANOVA test. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001