Fig. 1

TREM2 is highly expressed in the lesion region of the NMOSD mouse model. A Schematic diagram of AQP4-IgG purification (by Figdraw). B Schematic of experimental process in mouse model of NMOSD. C Representative images of western blotting analysis of TREM2 expression in sham and NMOSD group. D Quantification of TREM2 protein relative expression. Data is shown as fold change over the sham group. N = 4 mice/group, Mann–Whitney U test. E Representative images of Iba1 (red) and TREM2 (green) immunostaining in the striatum in sham and NMOSD mice. Scale bar, 30 μm. F Quantification of the ratio of TREM2⁺Iba1⁺/ Iba1⁺ cells in sham and NMOSD mice. N = 4 mice/group, n = 8 fields/group, Mann–Whitney U test