Fig. 4

TREM2 deletion inhibited microglia activation in the mouse model of NMOSD. A Representative images of Iba1 (red) and DAPI (blue) immunostaining in the striatum of WT and TREM2^−/− mice with or without AQP4-IgG and complement injection. Scale bar, 20 μm. B Representative images of Iba1 (red) and Ki67 (green) immunostaining in the striatum of WT and TREM2^−/− mice with or without AQP4-IgG and complement injection. Scale bar, 20 μm. C Quantification of the number of microglia per high-power field (HPF) in the striatum. N = 5 mice/group, n = 10 fields/group, two-way ANOVA with Bonferroni multiple comparisons. D Quantification of the ratio of Ki67⁺Iba1⁺/ Iba1⁺ cells. N = 5 mice/group, n = 10 fields/group, two-way ANOVA with Bonferroni multiple comparisons. E Representative images of the 3D reconstructions of Iba1⁺ cells. Scale bars, 20um. F Quantification of the soma volume, soma area, sphericity, and solidity of microglia in the striatum of WT and TREM2^−/− mice with or without AQP4-IgG and complement injection. N = 5 mice/group, n = 10–15 cells/mice, two-way ANOVA with Bonferroni multiple comparisons. G Representative images of the intersections masks of Sholl analysis (top) and quantification of the Sholl profiles for four groups (Bottom). Intersections were counted at 4 um intervals from the soma center to a radius of 41 um. N = 5 mice/group, n = 30 cells/mice, ****p < 0.0001, *p < 0.05, WT-NMOSD mice versus TREM2^−/−-NMOSD mice; ####p < 0.0001, ##p < 0.01, WT-Sham versus WT-NMOSD mice, two-way ANOVA with Bonferroni multiple comparisons. H Representative images of Iba1, CD68, Mac2 and MHC II stating at 7dpi. Scale bar, 20 μm. I Volume of CD68⁺ structures and percentages of Mac2⁺ and MHC II⁺ cells verse total Iba1⁺ cells. N = 5 mice for each group, n = 10 fields/group, two-way ANOVA with Bonferroni multiple comparisons