Fig. 10

Specific overexpression of CXCL13 in SCDH neurons induces mechanical allodynia in naïve mice. A Cartoon showing intra-dorsal horn microinjection of AAV9-hSyn-Cxcl13-EGFP or control AAV in naïve C57BL/6 mice. B Time schedule of the experiments. C Fluorescence images showing EGFP signal co-localized with Nissl (indicated by white arrowheads), but not with GFAP or Iba-1 in SCDH of AAV-injected mice. D Western blot showing an increased CXCL13 expression in SCDH from AAV-hSyn-Cxcl13-EGFP injected mice compared with control AAV-injected mice. E Specific overexpression of CXCL13 in SCDH neurons induced mechanical allodynia in mice. n = 4–6 mice/group. *p < 0.05, **p < 0.01. Student’s t test was used for comparisons in D. Two-way ANOVA with repeated measures followed by Tukey’s post hoc test was used for comparison in E