Fig. 7
Effects of IPA-3 (a PAK1 inhibitor) on COX-2 and PTGES2 protein levels and KA-induced CA3 neuronal damage in WT and PLPP/CINTg mice and effects of KA on p65 S276 and COX-2 level in PLPP/CIN−/− mice. PLPP/CIN overexpression enhances KA-induces COX-2 and PTGES2 upregulations and aggravates CA3 neuronal damage 3 days after KA injection, which are ameliorated by IPA-3 pretreatment. PLPP/CIN ablation does not influence KA-induced p65 S276 phosphorylation and COX-2 expression. a Representative Western blot data of COX-2 and PTGES2 in the whole hippocampi of WT and PLPP/CINTg mice b, c Quantifications of COX-2 and PTGES2 levels based on Western blot data (*p < 0.05 vs. WT mice, #p < 0.05 vs. control animals and $p < 0.05 vs. KA-treated animals with Newman-Keuls post-hoc test, n = 7, respectively). d Representative images for FJB-positive degenerating CA3 neurons at 3 days after KA injection in WT and PLPP/CINTg mice. e Quantitation of the number of FJB-positive CA3 neurons (*p < 0.05 vs. WT mice and #p < 0.05 vs. KA-treated animals with Newman-Keuls post-hoc test, n = 7, respectively). f Representative photos for p65 S276 phosphorylation and COX-2 expressions in CA3 neurons of PLPP/CIN−/− mice 2 h after KA injection