Fig. 4

Myeloid-resident HIF1α and IRE1α influence sterile inflammation. A RT-qPCR analysis of Vegfa, Tnf, Il1b, and Il6 mRNA expression in retinas from LysM-Hif1a^−/− and LysM-Ern1^−/− mice and their control LysM-cre/Hif1a^+/+/Ern1^+/+ mice conditions at P14 and B P17 under normoxia and OIR conditions. n = 5–8 retinas per condition. Results are shown as a fold change relative to respective normoxia control for each time point ± S.E.M. C–H LysM-Hif1a^−/−, LysM-Ern1^−/−, LysM-cre/Hif1a ^fl/f/Ern1^fl/fl mice and their control LysM-cre/Hif1a ^+/+/Ern1^+/+ mice were subjected to OIR, and retinas were collected at P14 and P17, flat-mounted, and stained with isolectin B4. C, D Representative photomicrographs of isolectin B4-stained LysM-Hif1a^−/−, LysM-Ern1^−/−, LysM-cre/Hif1a^fl/f/Ern1^fl/fl and LysM-cre/Hif1a^+/+/Ern1^+/+ mice at P14 with highlighted avascular hypoxic regions, and their quantification. E, F Representative photomicrographs of isolectin B4-stained LysM-Hif1a^−/−, LysM-Ern1^−/−, LysM-cre/Hif1a ^fl/f/Ern1^fl/fl and LysM-cre/Hif1a^+/+/Ern1^+/+ mice at P17 with highlighted pathological neovascularization, and their quantification. G, H Representative photomicrographs of isolectin B4-stained LysM-Hif1a^−/−, LysM-Ern1^−/−, LysM-cre/Hif1a ^fl/f/Ern1^fl/fl and LysM-cre/Hif1a^+/+/Ern1^+/+ mice at P17 with highlighted avascular hypoxic regions, and their quantifications. n = 5–13 retinas per group (C–H). Scale bars: 1 µm (for the whole flat mount of retina) and µm (for one petal of retina flat mount). Data expressed as mean ± S.E.M. Statistical analysis (A, B, D, F, H): one-way ANOVA with Bonferroni post hoc analysis; *P < 0.05, **P < 0.01, and ***P < 0.001