Fig. 2

c-Jun phosphorylation in the activated Bergmann glia of SCA1 mice. A Immunostaining of 16-week-old SCA1 mouse cerebellum with Bergmann glia (BG)-specific S100 (red) along with anti-c-Jun-pS63 antibody (green). Scale bar = 100 μm. White-boxed regions represent the corresponding higher-magnification images shown in the “zoom” panels. B HRP-based DAB immunostaining of SCA1 mouse cerebellum with anti-c-Jun-pS63 antibody. Scale bar = 50 μm. C Quantification of percentage of BG cells positive for c-Jun-pS63 stain shown in A. D Immunostaining of SCA1 mouse cerebellum with total c-Jun (green). Sections are counterstained with DAPI (blue) to detect nuclei. Scale bar = 100 μm. E Immunostaining of 16-week-old SCA1 mouse cerebellum with glia-specific GFAP antibody (green) along with calbindin antibody to detect Purkinje cells (red). Scale bar = 100 μm. F Quantification of GFAP intensity. BGL Bergmann glia layer; GCL granule cell layer; ML molecular layer. Sections are stained for nuclei using DAPI (blue). n = 4 mice. **P < 0.01; ***P < 0.001, 2-tailed unpaired Student’s t test