Fig. 1
Treatment with 1 μM Aβcore reduces astrogliosis and microgliosis in organotypic slice cultures from 7-month-old 5xFAD mice. Organotypic slice cultures from 1.5- and 7-month-old B6SJL (background) and 5xFAD mice were treated daily for 7 days with media only (Control) or 1 μM N-Aβcore. A Representative images of GFAP-positive astrocytes (green) and B Iba1-positive (green) / CD68-positive (red) microglia in the somatosensory cortex (top) and CA1 region of the hippocampus (bottom) from 1.5- and 7-month-old B6SJL and 5xFAD mice. The first pairs of 1.5-month-old Control B6JSL images were counterstained with DAPI (blue). Scale bar: 50 μm. Analysis of cell area per ROI 34,000 μm.2 for GFAP-positive astrocytes (C) and Iba1-positive microglia (D) (n = 20 cells across 6 slices from 3 mice for each condition). Number of processes per astrocyte (E) and microglia (F) (n = 20 cells across 6 slices from 3 mice per condition). Data are means ± SD. All data in C–E analyzed via two-way ANOVA with Tukey post hoc test as compared to Control for each treatment day. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001