Fig. 4
N-Aβcore attenuates synaptic loss induced by Aβ1–42. A Representative images of synapses, as determined by the colocalization of the synaptic protein Bassoon (green) and postsynaptic protein Drebrin (red), in primary cortical neurons from mixed neuronal/glia cultures after 5 days daily treatment with media only (Control), 1 μM Aβ1–42, 1 μM N-Aβcore, or 1 μM Aβ1–42 + 1 μM N-Aβcore. Bottom row: Magnified images of Bassoon-labeled presynaptic puncta (green) on Drebrin (red) labeled dendrite (white arrowhead). Images obtained on a Leica SP8 confocal microscope using a 63× objective. Scale bar: 50 μm. Primary antibodies [1:200 Mouse anti-Bassoon antibody (Abcam) and 1:200 Rabbit anti-Drebrin (Abcam)] were omitted in the secondary only sample. B The number of synaptic boutons per μM dendrite after treatments as described in A. (n = 9), where n represents the number of replicates. Data are means ± SD, analyzed via one-way ANOVA with Dunnett post hoc test as compared to 1 μM Aβ1–42