Fig. 1
From: Analysis of the microglia transcriptome across the human lifespan using single cell RNA sequencing
Isolation and characterization of human microglia. A Microglia were isolated from brain tissues at different ages, followed by tissue processing, single cell RNA sequencing and bioinformatics analysis. Three biological replicates per developmental stage (n = 12 in total) were used for downstream analysis. B UMAP plot of 13 clusters of 13,583 isolated microglia and relative size of each cluster. C UMAP plot representing the distinct expression profile of pre- compared to post-natal cells. Each age is represented by a separate color. D UMAP plot showing normalized expression of canonical microglia genes in all cells. E Stack bar plot showing the contribution of microglia from each age in each cluster. Different ages are represented by a distinct color