Fig. 2
From: Analysis of the microglia transcriptome across the human lifespan using single cell RNA sequencing
Microglia transcriptional clusters link to specific functions. A Heatmap of top 10 DEGs per cluster. Gene expression is represented by a color-coded z-score. Up and down regulated genes are shown by yellow and violet colors respectively. B Alluvial plot representing the most affected biological processes by upregulated genes of each cluster. Ribbon thickness indicates the number of genes per biological process. C UMAP plot highlighting three microglia clusters associated with inflammatory phenotypes. Stack bar plot showing contribution of each age group to these three clusters. Each age group is represented by a distinct color. Violin plot depicting expression of significantly upregulated gene in these three clusters *adjusted p-value < 0.05 (Wilcox test). D UMAP plot highlighting microglia originating from the second wave of microglia development. Pie chart showing the contribution of microglia of each age to cluster 13. Each age is represented by a distinct color. Violin plots showing expression of MS4A4A and MS4A7 genes * adjusted p-value < 0.05 (Wilcox test)