Fig. 5
From: Analysis of the microglia transcriptome across the human lifespan using single cell RNA sequencing
Pre-natal microglia have a distinct transcription factor activity profile compared to post-natal microglia. A Heatmap depicting top 5 microglia transcription factor signatures for each age group. Red and blue colors indicate up and down regulation of genes, respectively. B SCENIC plot depicting unbiased microglia clustering according to inferred TF activity separated by age. Each age group is represented by a distinct color. C, D SCENIC plot representing mRNA expression, binary regulon activity, and kernel density AUC histogram for ATF4 and E2F4 TF. Expression of genes is represented by a color gradient. TF activity is represented by a binary index in which blue denotes active and grey denotes inactive. E Density plot showing global activity map of TFs. Highly active and less active spots are represented by dark and light orange color respectively. F SCENIC plot representing mRNA expression and binary regulon activity of selected TFs. Expression of genes is represented by a color gradient. TF activity is represented by a binary index in which blue denotes active and grey denotes inactive