Fig. 1
From: Experimental hepatic encephalopathy causes early but sustained glial transcriptional changes
Isolation of astrocytes and microglia using FACS yields enriched astrocyte and microglial fractions. A Experimental set-up. B Representative flow cytometry plots indicating gating strategy used to identify microglia and astrocytes in all mice. C Relative abundance of microglia and astrocytes in sham and BDL mouse brains 14 and 28 days after induction. Data are pooled from 2 experiments with n = 12–18. D Heatmap visualizing log2TPM expression levels of cell-type specific markers of microglia, astrocytes, neurons, oligodendrocytes, oligodendrocyte precursor cells, pericytes and endothelial cells in isolated microglia and astrocytes. Heatmaps represent an average of n = 4–12 samples per group