Fig. 4

PA load in peripheral organs and brain regions. a Bacterial growth in lung and spleen (CFUs per mg of tissue) and brain (in CSF; CFU per μL) obtained from infected animals (OD₆₀₀ 0.3) and controls after intratracheal administration of PA at 24 h, 7-days and 1-month post-infection. b Representative confocal micrograph showing (b) lung and (c) spleen sections obtained from GFP-labelled PA infected animals at 24 h post-infection (OD₆₀₀ 0.3) and controls. PA is labelled in green and nuclei (DAPI) in blue. d–g Representative confocal micrographs of (d) cortical sections, (e) choroid plexus (f) hippocampus and (g) isolated capillaries showing the lack of distribution of PA (GFP-labelled PA) in the brain of infected mice at 24 h post-infection and controls. Lectin (red) was used to label the blood vessels in tissue sections and claudin-5 or ZO-1 (red) to label isolated capillaries. DAPI (blue) was used to stain the nuclei