Table 1 Primers used for reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis
Gene | Primer (5′–3′) | Annealing temperature (°C) | The size of production (bp) |
|---|---|---|---|
TNF-α | F: CCTCACCCACACCGTCAG R: GCAGGTCCCCCTTCTCCA | 60 °C | 170 bp |
TNFR1 TNFR2 | F: ATGTCGGAAAGAAATGTTC R: ATGCGTCTCACTCAGGTAG F: TAGGACTGGCGAACTGCT R: GACTCTTGCTTGGGGATG | 60 °C 60 °C | 109 bp 230 bp |
GAPDH | F: GTCGGTGTGAACGGATTTG R: TCCCATTCTCAGCCTTGAC | 60 °C | 181 bp |