Fig. 1

LCN2 in astrocytes is upregulated following ischemia/reperfusion injury both in vivo and in vitro. a, b Double-label IF staining of GFAP (green) and LCN2 (red) in the peri-infarct region and OGD/R-treated astrocytes. Scale bar = 20 μm. c–f Immunoblotting analysis of LCN2 expression after MCAO or OGD/R. Data are mean ± SD; n = 3 per group; ***P < 0.001, *P < 0.05 vs. sham-operated or control group; ^###P < 0.001, ^$$$P < 0.001