Fig. 2

Lcn2 knockout reduces infarct volume and neuronal death after MCAO surgery. a, b Infarct volume was assessed by TTC (2,3,5-triphenyltetrazoliumchloride) staining 24 h after the MCAO surgery (n = 10 per group). c, d Representative images of TUNEL double-staining (red) with NeuN (green) to assess the proportion of apoptotic neurons after the MCAO surgery. Scale bar = 50 μm. Data are mean ± SD; ***P < 0.001 vs. corresponding sham-operated group; ^###P < 0.001