Fig. 6

AAV-GFAP-24p3Ri transfection reduces 24p3R expression in astrocytes. a Schematic representation of the intracerebroventricular injection procedure. b Representative images of the small animal imaging system. A WT MCAO-operated mice. B WT MCAO-operated mice with AAV-GFAP-GFP injection. C WT MCAO-operated mice with AAV-GFAP-24p3Ri injection. c Representative images of AAV (labeled with GFP, green) specifically transfected into astrocytes but not microglia and neurons (labeled with GFAP, Iba-1 and NeuN, red). Scale bar = 20 μm. d qPCR was used to verify the efficacy of 24p3Ri at the transcriptional level. n = 3 per group; *P < 0.05 vs. vehicle-injection group; ^#P < 0.5. e, f Detection of 24p3R expression after interference at the translational level by WB. n = 3 per group; ***P < 0.01 vs. vehicle-injection group; ^###P < 0.001. g IF showed that astrocytic-24p3R expression was significantly inhibited by AAV-GFAP-24p3Ri injection. Scale bar = 20 μm