Fig. 2

VZV antigen specific response of human TG-derived CD8 T-cells. A, B TG-derived T-cell lines of donor TG04 (A) or TG07 (B) were screened for reactivity with artificial antigen presenting cells expressing the indicated subject-specific HLA class I allele(s) and individual VZV open reading frames or fragments thereof. For subject TG04, all 4 HLA alleles were co-transfected simultaneously, while for subject TG07, only HLA-A*11:01 was used. Empty vector (control) and phytohemagglutinin (PHA-P), were used as negative and positive controls, respectively. Levels of secreted IFNγ was determined by ELISA. Data are presented as the individual (circles) and mean (bars) OD₄₅₀ values of two independent replicates. Horizontal dashed lines indicate the threshold of T-cell response set at twice the value of the empty vector control. Viral ORF nomenclature according to VZV reference strain Dumas (Genbank Accession number X04370)