Fig. 3

TG-derived CD8 T-cells recognize an epitope in VZV ORF9 epitope in an HLA-A*11:01-dependent manner. A IFNγ secretion by TG07-derived T-cells following 24 h of incubation with Cos-7 cells co-transfected with a vector encoding HLA-A*11:01 and either an empty control vector or a vector encoding VZV ORF9. B Overview of the peptide composition of each ORF9 peptide pool used in panel C. Reactive pools are shaded. C IFNγ secretion by TG07-derived T-cells co-cultured with Cos-7 cells transfected with HLA-A*11:01 and pulsed with the indicated peptide pools, showing reactivity for two individual pools that overlap at peptide 28. D IFNγ secretion by TG07-derived T-cells following co-culture with HLA-A*11:01-transfected Cos-7 cells transfected and pulsed with ORF9 peptide 28 (amino acids 109–121, EDAVYENPLSVEK) or internal ORF9 peptide 28* (amino acids 111–121, AVYENPLSVEK). E Alignment of the VZV ORF9 epitope (AVYENPLSVEK, underlined) with the homologous regions of HSV-1 and HSV-2 UL49. Amino acids in bold differ between VZV and HSV-1/HSV-2; numbers indicate amino acid positions in the respective proteins. F IFNγ secretion by TG07-derived T-cells following co-culture with Cos-7 cells transfected with HLA-A*11:01 in combination with either an empty control vector or vectors encoding HSV-1 UL49 or HSV-2 UL49. Data are presented as the individual (circles) and mean (bars) OD₄₅₀ values of at two independent replicates