Fig. 5

No correlation between T-cell infiltrates and VZV latency in human TG. A Consecutive TG sections were stained for CD3 by immunohistochemistry (red; left column) or VLT RNA by in situ hybridization (dark red, right column). Filled and open arrow heads indicate CD3⁺ cells and a VLT⁺ neuron, respectively; the inset shows a VLT⁺ neuron. Data is shown for two different areas (1 and 2) of one donor that are representative of five independent TG donors; scale bar: 50 µm. B, C Abundance of VZV DNA (B) or VZV VLT and VZV VLT-ORF63 RNA (C) as well as human CD3D transcripts in 18 TG specimens (described in [3]) as determined by q(RT)-PCR and presented as relative DNA/transcript levels normalized to single-copy gene HMBS or cellular housekeeping gene GAPDH. Spearman r and p values are indicated