Fig. 7

Ogt depletion reduces the level of O-GlcNAcylation and induces activation of astrocytes derived from iPSCs. a qRT-PCR results showed that Ogt depletion significantly reduced the level of Ogt in astrocytes derived from iPSCs. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test. b–d qRT-PCR results showed that Ogt depletion significantly enhanced the level of Gfap (b), IL-1β (c) and TNF-α (d) in astrocytes derived from iPSCs. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test. e–g WB assay (e) and quantification results showed that Ogt depletion significantly enhanced the level of Ogt (f) and O-GlcNAcylation (g) in astrocytes derived from iPSCs. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test. h–j WB assay (h) and quantification results showed that Ogt depletion significantly enhanced the levels of of IL-1β (i) and TNF-α (j) in astrocytes derived from iPSCs. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test. k–n WB assay and quantification results showed that Ogt depletion did not affect the level of cytoplasmic p65 in astrocytes derived from iPSCs (k, l), but significantly increased the level of nuclear p-p65 (m, n). n = 4 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test