Fig. 1
ZIKV infection and proliferation in OHC. A NS1 fluorescence Intensity (FI) normalized by tissue area (DAPI +) (FI NS1/mm2) was measured for the two isolates 8, 24 and 48 h p.i.. Values outside the range between the lower and upper quartiles of the median of their respective groups (interquartile range) were excluded from the analysis. Two-way ANOVA test was used followed by Tukey’s multiple comparison test. Data were expressed as mean ± standard deviation. *P < 0.05, ***P < 0.01 and ****P < 0.0001. Number of animals = PE243 8 h (7), PE243 24 h (7), PE243 48 h (9), SPH 8 h (11), SPH 24 h (6), SPH 48 h (9). B Percentage of mature neurons infected with ZIKV (%NeuN + NS1 +) 8, 24 or 48 h p.i.. Values outside the interquartile range were excluded from the analysis. Two-way ANOVA test was used followed by Tukey’s multiple comparison test. Data were expressed as mean ± standard deviation. ****P < 0.0001. Sample size = PE243 8 h (6), PE243 24 h (7), PE243 48 h (9), SPH 8 h (8), SPH 24 h (6), SPH 48 h (8). C Micrographs (60 ×) obtained from OHC 8 h p.i.. Cell nuclei are stained in blue (DAPI), mature neurons in red (NeuN) and ZIKV in green (NS1). In detail, a digital zoom (2,5X) and 3D images show NS1 signals close to the cell nuclei, confirming its intracellular location. Scale bar = 10 µm