Fig. 2
PE243 and SPH2015 have differing effects on microglia in the OHC. A The areas corresponding to activated microglia (Iba1 +) were evaluated in mock-infected controls and after infection with ZIKV PE243 or SPH2015 at 8 h, 24 h and 48 h p.i.. Two-way ANOVA test was used followed by Tukey’s multiple comparison test. Statistical significance of P < 0.0001 for variations between rows and/or columns (e.g., effect of time, infection, and their interaction). Data were expressed as mean ± standard deviation of Iba1 + /mm2 z-scores calculated for the difference between the control and experimental groups in each endpoint. **P < 0.01, ***P < 0.001 and ****P < 0.0001. Number of animals = CTRL (6), PE243 (6), SPH (6) in each endpoint. B The means of microglia endpoints/branch length ratio were evaluated to verify microglia activation after infection with ZIKV PE243 or SPH2015 at 8 h, 24 h and 48 h p.i.. Two-way ANOVA test was used followed by Tukey’s multiple comparison test. Statistical significance of P < 0.0001 for variations between rows and/or columns (e.g., effect of time, infection, and their interaction). Data were expressed as mean ± standard deviation. *P < 0.05, **P < 0.01 and ****P < 0.0001. Number of animals = CTRL (3), PE243 (3), SPH (3) in each endpoint. C Representative micrographs (60 ×) obtained from OHC at 24 h p.i.. Cell nuclei are stained in blue (DAPI) and microglia in green (Iba1). Scale bar = 25 µm