Fig. 4
From: A role for P-selectin and complement in the pathological sequelae of germinal matrix hemorrhage
Microglial morphology and internalization of complement within the periventricular region after GMH. Morphological analysis of microglia from different experimental groups in terms of ramified vs. amoeboid characteristics. a Representative 63 × confocal anti-iba1 immunofluorescent imaging following deconvolution and reconstruction for Imaris microglia processing. b Representative microglia from vehicle treated GMH mouse showing amoeboid characteristics. c Representative microglia from 2.3Psel-Crry treated GMH mouse treated mouse showing more ramified morphology. d Representative image of low level C3 internalization in a ramified microglia from 2.3Psel-Crry treated GMH mice (left) and high level C3 internalization in more ameboid-like microglia from vehicle treated GMH mice (right). Various morphologic characteristics were quantified: e summation of processes length per microglia, f summation of number of processes per microglia, and g summation of number of processes terminal points per microglia. One-way ANOVA with Kruskal–Wallis correction (for non-parametric distribution) and Dunn’s multiple comparison. **p < 0.01, ****p < 0.0001. Error bars = Median with interquartile range. Internalization of complement deposition was quantified: h as total volume of internalized C3 and internalized h C3 volume within each microglia as a percentage of the individual microglia volume. One-way ANOVA with Kruskal–Wallis correction (for non-parametric distribution) and Dunn’s multiple comparison. **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars = Median with interquartile range