Fig. 7

Behavioral analysis of GMH mice and effect of 2.3Psel-Crry treatment. Nest Building task quantified by Deacon Nest Score (a) and weight of untorn nestlet square (b). T test with Mann–Whitney correction (for non-parametric distribution) and Dunn’s multiple comparison. *p < 0.05, **p < 0.01. Error bars = Median with interquartile range. c Elevated Plus Maze task quantified at P45 in terms of percent of time spent in open arms of maze. Increased stress and anxiety (more time in open arms) seen in vehicle treated GMH animals to 2.3Psel-Crry treated GMH animals. No difference between 2.3Psel-Crry treated and naïve mice. One-way ANOVA with Turkey’s correction. **p < 0.01, ***p < 0.001. Error bars = mean ± SEM. d Ultrasonic Vocalizations quantified by total number of calls when pups removed from mothers. Increased call number (increased stress and anxiety) in vehicle treated GMH mice compared to 2.3Psel-Crry treated and naïve mice at P11. Two-way ANOVA with Sidak’s correction for multiple comparisons. **p < 0.01, ****p < 0.0001. Error bars = mean ± SEM. e Contextual fear conditioning quantified at P45 by total percent of time freezing when re-exposed to an environment associated with a shock (no cue). Student’s t test. *p < 0.05. Error bars = mean ± SEM. f Cued fear conditioning quantified at P45 by total percent of time freezing after exposure to cue. Unpaired Student’s t test. Error bars = mean ± SEM