Fig. 4

PRG modulated Th17/Treg imbalance and promoted the frequency and function of Treg. A t-SNE plot showing the CD4 + TC subsets of CDLNs in scRNA-seq. B Dot plot showing the relative changes in cell ratios of CD4 + T cell subsets across the three groups. The numbers on the right indicate the Log2FC values of the cell ratios (EAU/Naive and PRG/EAU). C Bar chart showing the number of PRG-DEGs and EAU-DEGs in CD4 + TC subsets. D Rose diagram showing the number of up and downregulated rescue-DEGs in CD4 + TC subsets. E Bar chart showing the ratio of rescue-DEGs to EAU-DEGs in each CD4 + TC subset. F Venn diagram showing the interaction of downregulated EAU-DEGs and upregulated PRG-DEGs in Treg cells. G The heatmap showing the relative levels of specific genes among three groups. H Representative GO biological process and pathways enriched in upregulated rescue-DEGs in Treg cells. The flow cytometry histograms (left) and column charts (right) showing the level of TGFBR2 (I) and BACH2 (J) in CD4 + FOXP3 + Treg cells among three groups (n = 5/group). Significance in I, J was calculated using one-way ANOVA test; ***P < 0.001, ****P < 0.0001