Fig. 6
From: Progesterone attenuates Th17-cell pathogenicity in autoimmune uveitis via Id2/Pim1 axis
PRG attenuated Th17 differentiation and pathogenicity in EAU through Id2/Pim1 pathway. A Network plot showing the top 20 “rescue target genes” of Id2 by importance. B The heatmap showing the expression correlation of downregulated rescue-DEGs of Th17 cells and these genes related to T cell activation, Th17 cell differentiation, IL-17 signaling pathway in the Id2 module. C Venn diagram showing the screening of “rescue target genes” that significantly correlated with the level of Id2. The flow cytometry histograms (left) and column charts (right) showing the percentage of CD4 + IL-17A + Th17 cells (D) and the level of Pim1 + in Th17 cells (E) between IRBP1-20 or IRBP1-20 + HELI groups (n = 5/group). The flow cytometry histograms (left) and column charts (right) showing the level of Id2 (F) and Pim1 (G) in Th17 cells among control, IRBP1-20, IRBP1-20 + PRG and IRBP1-20 + PRG + HELI groups (n = 5/group). H The representative fundus images of Vehicle-AT and PRG-AT groups (left). The white arrows indicate inflammatory exudation and linear lesions. The column charts showing the clinical scores between two groups (n = 5/group, right). I The representative H&E-stained images of Vehicle-AT and PRG-AT groups (left). Scale bars: 50 μm. The black arrows indicate retinal folding. The column charts showing the histological scores between two groups (n = 5/group, right). Significance in D, E and H, I was calculated using two-tailed unpaired Student's t-test; significance in F, G was calculated using one-way ANOVA test; **P < 0.01, ***P < 0.001, ****P < 0.0001