Fig. 6

OLT1177 reduces astrogliosis and microgliosis reactivity induced by MPTP. a and b Representative glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor molecule 1 (Iba1) immunostaining in the striatum (St, a) and substantia nigra pars compacta (SNpc, b). Mice were treated with 200 mg/kg of OLT1177 (OLT) for 7 days. Saline was used as the vehicle. Positive signal appears in purple for Iba1 and brown color for GFAP. The area outlined in the box is shown in higher magnification in the inset. c–f GFAP and Iba1 stereological counting in the St (c and d) and in the SNpc (e and f). g–j GFAP and Iba1 immunoreactivity in the St (g and h) and in the SNpc (i and j). Scale bar: 500 µm. Data are represented as mean ± SEM. N = 5 for Sham + Saline and N = 7 for both MPTP groups. One-way ANOVA with Tukey’s post hoc correction was used to analyze differences between groups. *p < 0.05, **p < 0.01, and ***p < 0.001