Fig. 5

The assessment of the ambient RNAs in the non-neuronal groups before and after decontamination treatment (BCAS1). MG/Immu, microglia and other immune cell; Ast, astrocyte; Ol, oligodendrocyte; OPC, oligodendrocyte precursor cell; FB, fibroblast; EndoOther, endothelial cell and other cell. A t-SNE plots show the enrichment of ambient RNA markers before and after decontamination treatment in different cell types by using irGSEA analysis with the Ucell algorithm. B Box plots show the comparisons of irGSEA density of ambient RNA markers before and after decontamination in different cell types. ****P < 0.0001. C Volcano plot shows DEGs of MG/Immu between the sham and BCAS groups after decontamination. D Dot plots show the pathways enrichment of up-regulated genes of MG/Immu in the BCAS group (C). E Venn plot shows the overlap of up-regulated genes of MG/Immu in the BCAS group before and after decontamination. F Dot plots show the pathways enrichment of 1453 up-regulated genes only identified before decontamination, but not in after decontamination