Fig. 6

The integrative analysis of single-nuclei and bulk cortex-specific transcriptome changes after chronic deeper cerebral hypoperfusion. Neun, neuron; MG/Immu, microglia and other immune cell; Ast, astrocyte; Ol, oligodendrocyte; OPC, oligodendrocyte precursor cell; FB, fibroblast; EndoOther, endothelial cell and other cell. A Volcano plot shows DEGs of bulk transcriptome between the BCAS and sham groups. B, C Dot plots show the pathways enrichment of up- and down-regulated genes in the bulk transcriptome data (BCAS vs sham, GSE210666). D, G Heatmaps show snRNA-seq expression of “Bulk_up_genes” and “Bulk_down_genes” in different cell types, respectively. “Bulk_up_genes” and “Bulk_down_genes” identified in bulk transcriptome data as shown in A. E, H t-SNE plots show the enrichment of snRNA-seq for “Bulk_up_genes” and “Bulk_down_genes” by using irGSEA analysis with the Ucell algorithm, respectively. F Bar plots show the enrichment between “Bulk_up_genes” and top 200 characteristic genes in each cell type of snRNA-seq data by using GeneOverlap. I Bar plots show the enrichment between “Bulk_down_genes” and top 200 characteristic genes in each cell type of snRNA-seq data by using GeneOverlap