Fig. 7

Subgroup analysis of MG/Immu of sham/BCAS mice at single-nuclei resolution. In our study, MG1, microglia 1; MG2, microglia 2; Apoe⁺ MG/Mac: Apoe⁺ microglia/macrophage; Mac2: macrophage; DC, dendritic cell. In published data (GSE189432), Micro3, microglia 3; Tcir, circulating T cells; CAM2, central nervous system-associated macrophages2; DC, dendritic cell. A t-SNE plots show all single-nuclei of MG/Immu in all samples. B Dot plots show the average expression of specific genes in different groups. C t-SNE plots show all single-nuclei of MG/Immu in the sham and BCAS groups, respectively. D Violin plots show the expression of Syt1 and Grin2b in different subgroups of MG/Immu. E t-SNE plots show the enrichment of MG/Immu subgroup in our snRNA-seq data for marker genes of Micro3 (GSE189432) by using irGSEA analysis with the AUcell algorithm. F t-SNE plots show the enrichment of MG/Immu subgroup in our snRNA-seq data for marker genes of Tcir (GSE189432) by using irGSEA analysis with the AUcell algorithm. G t-SNE plots show the enrichment of MG/Immu subgroup in our snRNA-seq data for marker genes of CAM2 (GSE189432) by using irGSEA analysis with the AUcell algorithm. H t-SNE plots show the enrichment of MG/Immu subgroup in our snRNA-seq data for marker genes of DC (GSE189432) by using irGSEA analysis with the AUcell algorithm