Fig. 3

Divergent transcriptomic profiles and expression of GRP78 in the hippocampus of anti-DWEYS and Ctl mice. A Volcano plot showing the DEGs between anti-DWEYS and Ctl group. The horizontal dotted line corresponds to a p of 0.05. Down-regulated genes are shown in blue dots and up-regulated in red. B Left, western blot showing GRP78 expression levels in hippocampus of anti-DWEYS and Ctl mice. Right, densitometric quantification of GRP78 in different groups (n = 3). C KEGG pathway analysis of DEGs in up-regulated and down-regulated expression. D Heatmap showing the expression patterns of up-regulated DEGs in “protein processing in endoplasmic reticulum” pathway. E Double staining shows that GRP78 (green) is co-localized with NeuN (red, left), but not with GFAP (red, middle), or Iba1 (red, right). F Representative immunofluorescence staining for NeuN (red) and IgG (green) in N2a cells under anti-DWEYS or Isotype IgG stimulation. G Cell viability determined by CCK-8 assay (n = 3). Data are mean ± SEM. ns, non-significant; **p < 0.01; One-way ANOVA and Dunnett’s multiple comparisons test. H Quantitative RT-PCR analysis of GRP78 in N2a cell lysate (n = 3). ***p < 0.001, t-test. I Comparisons of GRP78 protein levels in the lysate of N2a cells after being stimulated by anti-DWEYS or Isotype IgG. **p < 0.01, t-test. J Comparisons of GRP78 levels in the supernatant of N2a cells after stimulated by anti-DWEYS or Isotype IgG. ***p < 0.001, t-test