Fig. 6

Effects of rapamycin (rapa) on the neuropathic changes in anti-DWEYS mice. A Comparisons of GRP78 level in the supernatant of N2a cells upon anti-DWEYS IgG stimulation with or without rapa pretreatment (n = 6). B Volcano plot showing the DEGs between anti-DWEYS and anti-DWEYS + rapa groups. C Left, representative western blotting images of GRP78 in anti-DWEYS and anti-DWEYS + rapa mice. Right, densitometric quantification of GRP78 in different groups (n = 3). **p < 0.01, t test. D KEGG pathway analysis of DEGs in up-regulated and down-regulated expression between anti-DWEYS and anti-DWEYS + rapa groups. E Heatmap showing the expression patterns of down-regulated DEGs in “protein processing in endoplasmic reticulum pathway”. F Left, immunofluorescence staining for Iba-1 in the hippocampus. Right, quantification of Iba-1⁺ cells. G Comparisons of IL-6, TNF-α, and IL-1β from brain homogenates in different groups (n = 6). H Performance in the OPM task of anti-DWEYS and anti-DWEYS + rapa mice (n = 4). Data are mean ± SEM. ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; t test