Fig. 1

Bone marrow-derived macrophages (BMMs), endothelial progenitor cells (EPCs), and exosomes (EPC-EXOs) extraction and identification. A Immunocytochemical identification of primary mice bone marrow macrophages. Scale bar: 100 µm. B The bone marrow-derived EPCs were further defined by their ability to bind FITC-UEA-l and uptake Dil-ac-LDL. Scale bar: 100 μm. C Immunostaining revealed that the adherent cells were positive for CD133 and VEGFR2. Scale bar: 50 μm. D A schematic diagram of the extraction of EPC-EXOs. E Transmission electron microscopy (TEM) image of EPC-EXOs. Scale bar: 100 nm. F Nanoparticle tracking analysis (NTA) of EPC-EXOs diameter and particle number. G Immunoblotting of cell marker Calnexin and exosomal surface markers CD63, CD9, and TSG101