Fig. 6

MiR-222-3p promotes anti-inflammatory macrophages via the SOCS3/JAK2/STAT3 signaling pathway. A Schematic diagram of miR-222-3p and SOCS3′s 3′UTR binding sites. B Dual-luciferase reporter assays were performed to determine the targeting sequence of miR-222-3p and 3′UTR of SOCS3. C Western Blotting analysis of the expression levels of SOCS3 in macrophages with miR-222-3p-Mimic and Mimic NC. D Statistical analysis of the expression levels of SOCS3, n = 3, ***P < 0.001. E qRT-PCR analysis of mRNA expression of SOCS3 in macrophages with miR-222-3p-Mimic, Mimic NC, Inhibitor and Inhibitor NC, n = 3, ^nsP > 0.05, **P < 0.01. F Western blot assay was used to detect expression levels of SOCS3, iNOS and Arg-1 after co-transfection. G–I Statistical analysis of the expression levels of SOCS3, iNOS and Arg-1, n = 3, ***P < 0.001, ****P < 0.0001. J Western Blotting analysis of the expression levels of JAK2, p-JAK2, STAT3 and p-STAT3 in macrophages with miR-222-3p-Mimic, Mimic NC, Inhibitor and Inhibitor NC. K–N Statistical analysis of the expression levels of JAK2, p-JAK2, STAT3 and p-STAT3, n = 3, ^nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001