Fig. 4

Reduced activation of astrocytes and microglia in the spinal cord of SAFit2-treated animals. Immunohistochemistry staining of astrocyte activation marker GFAP (A) and microglia marker IBA1 (B) at 20X magnification (scale bar: 50 µm) of spinal cord (dorsal horn) slices. Samples of naïve animals were labeled as control. C Quantification of GFAP-positive cells. D Quantification of IBA1-positive cells. C, D Positive cells were counted in 15–20 images per animal and averaged. Data represent the mean ± SEM from 15–20 images of four mice per group. ** p < 0.01, *** p < 0.001, **** p < 0.0001 one-way ANOVA with Tukey’s multiple comparison test. SAFit2 selective antagonist of FKBP51 by induced fit 2, GFAP glial fibrillary acidic protein, IBA1 ionized calcium-binding adaptor molecule 1