Fig. 6
Influence of SAFit2 on phospholipase 2 activity in primary sensory neuron lysates. A Schematic illustration of the phospholipase 2 function and its products. Phospholipase 2 hydrolyzes the second ester bond of phospholipids that constitute the lipid bilayer of cell membranes. Thereby, polyunsaturated fatty acids were released, which were rapidly metabolized into oxylipins. B The influence of SAFit2 (0.1–50 µM) on the phospholipase 2 activity was assessed in lysed primary sensory neurons. Further, the basal phospholipase activity of the lysates was determined, the influence of the solvent DMSO (vehicle) verified and bee venom used as a positive control. Data represent the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 one-way ANOVA with Tukey’s post-multiple comparison test. PLA2 phospholipase 2, SAFit2 selective antagonist of FKBP51 by induced fit 2