Fig. 3

Knockdown of CD200 in DG region facilitates stress susceptibility of mice. A Experimental timeline for LV-siCD200 stereotaxical injection, SSDS procedure, and behavioral testing. B Representative image showing the expression of LV-siCD200 in DG 3 weeks after virus vector injection. Scale bar: 350 μm. C Immunoblot analysis of CD200 protein expression in the DG region of mice injected with LV-siCD200 (n = 3 per group, Student’s t test). D Heatmap of SIT in mice injected with LV-siCD200 or LV-GFP to DG, together with or without exposure to SSDS. E Social interaction time in the SIT of mice injected with LV-siCD200 and exposed to SSDS (n = 12–14 per group, one-way ANOVA with Fisher’s LSD test). F Immobility time in the TST of mice injected with LV-siCD200 and exposed to SSDS (n = 13–16 per group, two-way ANOVA with Fisher’s LSD test). Data are expressed as mean ± SEM. ns: not statistically significant; *P < 0.05; **P < 0.01. Ctrl, control. See Additional file 1: Table S2 for detailed statistical information