Fig. 4

CD200 alleviates CSDS-induced depressive-like behaviors through binding to CD200R1 on microglia. A Representative immunostaining for CD200R1 (green)/Iba1 (red)/DAPI (blue) in the DG. Scale bars: 60 μm (right), 30 μm (left). B Experimental timeline for LV-siCD200R1 stereotaxically injection, CSDS procedure, CD200Fc administration and behavioral testing. C Representative image showing the expression of LV-siCD200R1 expression in DG 3 weeks after virus vector injection. Scale bar: 350 μm. D Immunoblot analysis of CD200R1 protein expression in the DG region of mice injected with LV-siCD200R1 (n = 3 per group, Student’s t test). E Social interaction ratio in the SIT of control and CSDS mice injected with LV-siCD200R1 or LV-GFP (n = 7–9 per group, two-way ANOVA with Fisher’s LSD test). F Analysis of social interaction ratio in the SIT of control and CSDS mice injected with LV-siCD200R1 and CD200Fc (The CD200Fc administration group: n = 7–10 per group; The vehicle administration group: n = 6–11 per group, one-way ANOVA with Fisher’s LSD test). G Analysis of immobility time in TST of control and CSDS mice injected with LV-siCD200R1 and CD200Fc (The CD200Fc administration group: n = 8–11 per group; The vehicle administration group: n = 6–11 per group, one-way ANOVA with Fisher’s LSD test). H Analysis of immobility time in FST of control and CSDS mice injected with LV-siCD200R1 and CD200Fc (The CD200Fc administration group: n = 7–11 per group; The vehicle administration group; n = 6–11 per group, one-way ANOVA with Fisher’s LSD test). I Analysis of total distance in OFT of control and CSDS mice after injected with LV-siCD200R1 and CD200Fc (The CD200Fc administration group: n = 8–11 per group; The vehicle administration group; n = 6–11 per group, one-way ANOVA with Fisher’s LSD test). Data are expressed as mean ± SEM. ns: not statistically significant; *P < 0.05; **P < 0.01; ***P < 0.001. Ctrl, control. See Additional file 1: Table S2 for detailed statistical information