Fig. 1
From: Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function
Characterization of CART peptide (CARTp) antibodies. A PCR amplification curves of CART mRNA extracted from donor cadaver DMN and celiac ganglion tissues. CARTp mRNA was detected in the DMN, but not in the celiac ganglion. B Representative capillary westerns showing CARTp immunoreactivity for three commercially available CART antibodies. Synthetic CARTp was denatured and loaded as the sample. C Representative capillary westerns showing the reactivity of the mouse anti-CARTp antibody against different amounts of synthetic CARTp and scrambled CARTp. D Representative images of the paraventricular nucleus (PVN, a site known to contain CART-positive axons) showing CARTp immunoreactivity in tissue sections incubated with either the mouse anti-CART antibody (CART Ab), the mouse anti-CART antibody after overnight neutralization with 30 molar excess CART peptide (CART Ab + CARTp), or the mouse anti-CART antibody after overnight neutralization with 30 molar excess scrambled CART peptide (CART Ab + Scr pep)