Fig. 2
From: Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function
CART peptide (CARTp) is expressed in neurons in the caudal DMN. A Drawing of a coronal section of the human brain stem demonstrating the relative position of the DMN (rostral aspect in red) compared to the NTS and the 4th ventricle. B Representative images showing that CARTp immunoreactivity can be detected in neurons in the caudal, but not rostral, aspect of the DMN in humans. The insets show that both CARTp-positive cell somas (arrows) and fibers (arrowheads) can be seen in caudal DMN. C Representative mouse brain images showing CARTp immunoreactivity in neurons resident to the caudal, but not rostral, aspect of the DMN. D Intracerebroventricular administration of CART siRNA to mice significantly reduced CARTp immunoreactivity in the caudal DMN compared to control (non-targeting) siRNA. E Representative double-label immunohistochemistry showing co-localization of CARTp and ChAT immunoreactivities in the caudal DMN neurons in mice. Double immunostained cells appear yellow in the merged image