Fig. 3
From: Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function
CARTp immunoreactivity is detected in the mouse spleen. A Representative confocal images showing CARTp immunoreactivity in the spleen. CARTp immunoreactivity appears as both fibers (inset, arrows) and puncta and could be found along the longitudinal axis of the spleen. B Representative photomicrographs showing that CARTp and ChAT immunoreactivities colocalize in fibers that appear primarily along the boundary of the red pulp (RP) adjacent to the white pulp (WP). C CARTp-immunoreactive fibers (arrows) co-localized with the axonal marker neurofilament H (NF-H). D Drawing illustrating the relative position of the subdiaphragmatic vagotomy. E Representative images showing that four weeks following subdiaphragmatic vagotomy CARTp immunoreactivity in the spleen is lost