Fig. 6
From: Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function
CRISPR-Cas deletion of the Cartpt gene from the DMN exacerbates LPS-induced systemic inflammation. A Representative photomicrographs of saCAS9 (a RNA-guided DNA endonuclease) and CARTp immunoreactivities in the caudal DMN of mice injected with either Cartpt-CRISPR or a control AAV. Cartpt-CRISPR effectively reduced CART expression in the caudal DMN and B the spleen as compared to mice receiving the control AAV. C Summary data showing the circulating levels of IL-1ß in mice receiving Cartpt-CRISPR or control AAV infusions into the caudal DMN followed by injection of LPS (or vehicle). Loss of CART exacerbated circulating IL-1ß levels