Fig. 2

Effect of stroke on ovarian steroid hormone expression in adults and RS animals. LC–MS analysis of testosterone (A), progesterone (B), estrone (C), estradiol (D), androstenedione (E), 17-OH progesterone (F) and the ratio between estradiol and testosterone (G) in Adult and RS sham and stroke ovaries. H ELISA analysis of activin in Adult and RS sham and stroke ovaries. All samples were collected 2 days post-stroke, and a two-way ANOVA for age and stroke condition was used to compare groups. N = 7 Adult stroke, N = 6 Adult sham, N = 10 RS sham, N = 5 RS stroke. a Effect of stroke, b effect of age, c interaction effect. *p ≤ 0.05