Fig. 7

Macrophage flow cytometry analysis of brains and lymph nodes after ovariectomy. A Flow cytometry analysis of the brain of CD68 + (i) CD80 + (ii), CD115 + (iii), and MHCII + (iv) cells in RS intact and OVX females. B Flow cytometry analysis of the cervical lymph nodes of CD68 + (i) CD80 + (ii), CD115 + (iii), and MHCII + (iv) cells in RS intact and OVX females. C Flow cytometry analysis of the para-aortic lymph nodes of CD68 + (i) CD80 + (ii), CD115 + (iii), and MHCII + (iv) cells in RS intact and OVX females. D Flow cytometry analysis of the mesenteric lymph nodes of CD68 + (i) CD80 + (ii), CD115 + (iii), and MHCII + (iv) cells in RS intact and OVX females. Ei Heatmap analysis of immune cells populations in RS OVX and intact cervical, para-aortic, mesenteric lymph and brain post-stroke. Eii Sparse partial least squares discriminant analysis of macrophage populations in RS OVX and intact cervical, para-aortic, mesenteric lymph and brain post-stroke. All samples were collected 2 days post-stroke, and Student’s t test was used to compare groups. N = 7 (intact), N = 8 (OVX). *p ≤ 0.05