Fig. 8

T cell flow cytometry analysis of brains and lymph nodes after ovariectomy. A Flow cytometry analysis of the brain of CD3 + (i) CD4 + (ii), CD8 + (iii), CD25 + (iv), FoxP3 + (v), and TCRGD + (vi) cells in RS intact and OVX females. B Flow cytometry analysis of the cervical lymph nodes of CD3 + (i) CD4 + (ii), CD8 + (iii), CD25 + (iv), FoxP3 + (v), and TCRGD + (vi) cells in RS intact and OVX females. C Flow cytometry analysis of the para-aortic lymph nodes of CD3 + (i) CD4 + (ii), CD8 + (iii), CD25 + (iv), FoxP3 + (v), and TCRGD + (vi) cells in RS intact and OVX females. D Flow cytometry analysis of the mesenteric lymph nodes of CD3 + (i) CD4 + (ii), CD8 + (iii), CD25 + (iv), FoxP3 + (v), and TCRGD + (vi) cells in RS intact and OVX females. Eii Sparse partial least squares discriminant analysis of macrophage populations in RS OVX and intact cervical, para-aortic, mesenteric lymph and brain post-stroke. All samples were collected 2 days post-stroke, and Student’s t test was used to compare groups. N = 7 (intact), N = 8 (OVX). *p ≤ 0.05